bacterial
bacteria, pathogen, infection @ Pixabay

The acid-fast stain is a bacterial cell staining technique that allows for the visualization of certain bacteria. The cells are stained with an acidic compound, and then treated with a dye which only colors those cells that contain lipids in their cytoplasmic membranes. This technique can be used to identify Mycobacterium tuberculosis, as well as other acid-fast bacteria such as Nocardia species and some strains of Brucella species. The acid-fast stain is a bacterial cell staining technique that allows for the visualization of certain bacteria. The cells are stained with an acidic compound, and then treated with a dye which only colors those cells that contain lipids in their cytoplasmic membranes. This technique can be used to identify Mycobacterium tuberculosis, as well as other acid-fast bacteria such as Nocardia species and some strains of Brucella species. One limitation of this stain is its inability to visualize Gram negative organisms or nonlipid containing microbes on the Gram stained slide due to overlapping techniques. These limitations make it important not use this stain alone when determining whether a pathogen may cause disease; rather, it should be

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